Reporter

Part:BBa_K1891007:Design

Designed by: Zhao Jingyu   Group: iGEM16_BNU-China   (2016-10-10)


alpha-tubulin+YNE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 172
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We connected this two gene fragments using fusion PCR. First we acquired alpha-tubulin gene fragment and YNE gene fragment. And then we redesigned a antisense primer ofalpha-tubulin and a sense primer of YNE with a linker about 42bp. And then doing fusion PCR.


Source

Alpha-tubulin comes from human breast cancer cell line Mcf-7. YNE comes from pSPYNE plasmid.

References